肉用山羊脂代谢相关基因与肌内脂肪含量的相关性分析

doi:10.11843/j.issn.0366-6964.2016.07.005

摘要/Abstract

摘要：

本研究旨在鉴定脂代谢相关基因在不同肉用山羊品种肌肉组织中的表达水平，并分析其与肌内脂肪（IMF）含量的相关性，从而为研究山羊肌肉IMF的形成机制奠定基础。选取健康的简州大耳羊和成都麻羊成年羯羊各6只，屠宰后分别采集背最长肌、后腿股二头肌及臂三头肌，利用实时荧光定量PCR技术检测脂代谢相关基因的表达水平，同时测定肌肉中IMF的含量，分析基因表达水平与IMF含量之间的相关性。结果表明，除PPARG外，脂代谢相关基因（包括SREBP1c、THRSP、INSIG1、ACACA、SCD1、DGAT1和DGAT2）在简州大耳羊各肌肉组织中的mRNA表达量普遍高于成都麻羊，其中THRSP、SCD1、DGAT2的表达量在各组织中均显著高于成都麻羊（P<0.05）。除ACACA外，简州大耳羊背最长肌的基因表达量均显著高于其他两种组织，而后腿股二头肌和臂三头肌间差异均不显著（P>0.05）（除PPARG外）。除THRSP和SCD1在背最长肌中具有更高的mRNA表达外（P<0.05），成都麻羊脂代谢相关基因表达水平在不同肌肉组织中均无显著差异（P>0.05）。简州大耳羊各组织中的IMF含量均显著高于成都麻羊（P<0.05），背最长肌在不同山羊品种间均具有最高的IMF含量（P<0.05）。THRSP、DGAT2和SCD1在成都麻羊和简州大耳羊3个不同部位肌肉组织中的表达与IMF含量均存在显著正相关。这些数据表明，THRSP、SCD1和DGAT2在肉用山羊肌肉IMF沉积过程中具有重要的调控作用，也为进一步揭示IMF形成调控机制奠定了基础。

Abstract:

The aim of this study was to determine the mRNA expression of genes related to lipid metabolism in muscle tissue of different goat breeds，and to analyze their association with intramuscular fat (IMF) content，which was predicted to provide basic data for revealing the mechanism underlying IMF formation.Longissimus dorsi，biceps femoris muscle and triceps brachii muscle from 6 healthy castrated Jianzhou Big-eared goats and Chengdu Brown goats after slaughter，respectively，were collected for detecting IMF content，and the real-time PCR was used to detect the mRNA expression of genes related to lipid metabolism，and their association was analyzed.The result showed that，except for PPARG，the other lipid metabolism related genes(including SREBP1c， THRSP， INSIG1，ACACA，SCD1，DGAT1 and DGAT2) were expressed higher in Jianzhou Big-eared goat than in Chengdu Brown goat.Among them，the expression of THRSP，SCD1 and DGAT2 were significantly higher in Jianzhou Big-eared goat than in Chengdu Brown goat in different tissues (P<0.05).Except for ACACA，the expression of other lipid metabolism related genes were the highest in longissimus dorsi of Jianzhou Big-eared goat，with no significant difference between biceps femoris muscle and triceps brachii muscle (P>0.05) (except for PPARG).While the mRNA expression of THRSP and SCD1 were the highest in longissimus dorsi (P<0.05)，the lipid metabolism related genes had no significant differences between biceps femoris muscle and triceps brachii muscle in Chengdu Brown goat (P>0.05).Jianzhou Big-eared goat had higher IMF content than Chengdu Brown goat in all the 3 tissues (P<0.05)，longissimus dorsi had higher IMF content compared with the other tissues.THRSP，DGAT2 and SCD1 showed positive association with IMF in various tissues of Jianzhou Big-eared goat and Chengdu Brown goat.These results indicate that THRSP，SCD1 and DGAT2 may play important role in promoting fat deposition in meat goat，and also provide basic data for revealing the mechanism underlying goat IMF formation.

中图分类号:

S827
S813.3

朱武政，林亚秋，江明锋，王永，廖红海，李倩，朱江江. 肉用山羊脂代谢相关基因与肌内脂肪含量的相关性分析[J]. 畜牧兽医学报, 2016, 47(7): 1333-1341.

ZHU Wu-zheng，LIN Ya-qiu，JIANG Ming-feng，WANG Yong，LIAO Hong-hai，LI Qian，ZHU Jiang-jiang. Association Analysis of Intramuscular Fat Content and the Expression of Genes Related to Lipid Metabolism in Meat Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1333-1341.

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